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broad spectrum sstr antagonist tocris cat#3493  (Tocris)


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    Tocris broad spectrum sstr antagonist tocris cat#3493
    Broad Spectrum Sstr Antagonist Tocris Cat#3493, supplied by Tocris, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 1 article reviews
    broad spectrum sstr antagonist tocris cat#3493 - by Bioz Stars, 2026-03
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    rgds peptide (tocris bioscience; cat#3498)  (Tocris)
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    A) Representative immunoblot for pAKT (S473), AKT and GAPDH in TC11 shCtrl and shSEMA7A (KD#1 and KD#2) cell lines. Bar graph: ratio of pAKT to total AKT. Ordinary one-way ANOVA with Tukey’s multiple comparisons test. B) Representative immunoblot for pAKT (S473), AKT and GAPDH in TC11 shCtrl and shSEMA7A KD#2 cell lines after treatment for 24 hours with 20 μg/mL purified rSEMA7A, or conditioned media from SEMA7A OE cells (S7OE CM). Bar graph: ratio of pAKT to total AKT. Ordinary one-way ANOVA with Tukey’s multiple comparisons test. C) Representative immunoblot for pAKT (S473), AKT and GAPDH in TC11 WT cell lysates after treatment for 1 hour with 20 μg/mL rSEMA7A, 50 μM <t>RGDS</t> <t>peptide,</t> or both. Bar graph: ratio of pAKT to total AKT. Ordinary one-way ANOVA with Tukey’s multiple comparisons test. Error bars are mean +/- SD. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.
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    A) Representative immunoblot for pAKT (S473), AKT and GAPDH in TC11 shCtrl and shSEMA7A (KD#1 and KD#2) cell lines. Bar graph: ratio of pAKT to total AKT. Ordinary one-way ANOVA with Tukey’s multiple comparisons test. B) Representative immunoblot for pAKT (S473), AKT and GAPDH in TC11 shCtrl and shSEMA7A KD#2 cell lines after treatment for 24 hours with 20 μg/mL purified rSEMA7A, or conditioned media from SEMA7A OE cells (S7OE CM). Bar graph: ratio of pAKT to total AKT. Ordinary one-way ANOVA with Tukey’s multiple comparisons test. C) Representative immunoblot for pAKT (S473), AKT and GAPDH in TC11 WT cell lysates after treatment for 1 hour with 20 μg/mL rSEMA7A, 50 μM <t>RGDS</t> <t>peptide,</t> or both. Bar graph: ratio of pAKT to total AKT. Ordinary one-way ANOVA with Tukey’s multiple comparisons test. Error bars are mean +/- SD. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.
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    A) Representative immunoblot for pAKT (S473), AKT and GAPDH in TC11 shCtrl and shSEMA7A (KD#1 and KD#2) cell lines. Bar graph: ratio of pAKT to total AKT. Ordinary one-way ANOVA with Tukey’s multiple comparisons test. B) Representative immunoblot for pAKT (S473), AKT and GAPDH in TC11 shCtrl and shSEMA7A KD#2 cell lines after treatment for 24 hours with 20 μg/mL purified rSEMA7A, or conditioned media from SEMA7A OE cells (S7OE CM). Bar graph: ratio of pAKT to total AKT. Ordinary one-way ANOVA with Tukey’s multiple comparisons test. C) Representative immunoblot for pAKT (S473), AKT and GAPDH in TC11 WT cell lysates after treatment for 1 hour with 20 μg/mL rSEMA7A, 50 μM <t>RGDS</t> <t>peptide,</t> or both. Bar graph: ratio of pAKT to total AKT. Ordinary one-way ANOVA with Tukey’s multiple comparisons test. Error bars are mean +/- SD. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.
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    A) Representative immunoblot for pAKT (S473), AKT and GAPDH in TC11 shCtrl and shSEMA7A (KD#1 and KD#2) cell lines. Bar graph: ratio of pAKT to total AKT. Ordinary one-way ANOVA with Tukey’s multiple comparisons test. B) Representative immunoblot for pAKT (S473), AKT and GAPDH in TC11 shCtrl and shSEMA7A KD#2 cell lines after treatment for 24 hours with 20 μg/mL purified rSEMA7A, or conditioned media from SEMA7A OE cells (S7OE CM). Bar graph: ratio of pAKT to total AKT. Ordinary one-way ANOVA with Tukey’s multiple comparisons test. C) Representative immunoblot for pAKT (S473), AKT and GAPDH in TC11 WT cell lysates after treatment for 1 hour with 20 μg/mL rSEMA7A, 50 μM <t>RGDS</t> <t>peptide,</t> or both. Bar graph: ratio of pAKT to total AKT. Ordinary one-way ANOVA with Tukey’s multiple comparisons test. Error bars are mean +/- SD. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.
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    A) Representative immunoblot for pAKT (S473), AKT and GAPDH in TC11 shCtrl and shSEMA7A (KD#1 and KD#2) cell lines. Bar graph: ratio of pAKT to total AKT. Ordinary one-way ANOVA with Tukey’s multiple comparisons test. B) Representative immunoblot for pAKT (S473), AKT and GAPDH in TC11 shCtrl and shSEMA7A KD#2 cell lines after treatment for 24 hours with 20 μg/mL purified rSEMA7A, or conditioned media from SEMA7A OE cells (S7OE CM). Bar graph: ratio of pAKT to total AKT. Ordinary one-way ANOVA with Tukey’s multiple comparisons test. C) Representative immunoblot for pAKT (S473), AKT and GAPDH in TC11 WT cell lysates after treatment for 1 hour with 20 μg/mL rSEMA7A, 50 μM <t>RGDS</t> <t>peptide,</t> or both. Bar graph: ratio of pAKT to total AKT. Ordinary one-way ANOVA with Tukey’s multiple comparisons test. Error bars are mean +/- SD. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.
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    A) Representative immunoblot for pAKT (S473), AKT and GAPDH in TC11 shCtrl and shSEMA7A (KD#1 and KD#2) cell lines. Bar graph: ratio of pAKT to total AKT. Ordinary one-way ANOVA with Tukey’s multiple comparisons test. B) Representative immunoblot for pAKT (S473), AKT and GAPDH in TC11 shCtrl and shSEMA7A KD#2 cell lines after treatment for 24 hours with 20 μg/mL purified rSEMA7A, or conditioned media from SEMA7A OE cells (S7OE CM). Bar graph: ratio of pAKT to total AKT. Ordinary one-way ANOVA with Tukey’s multiple comparisons test. C) Representative immunoblot for pAKT (S473), AKT and GAPDH in TC11 WT cell lysates after treatment for 1 hour with 20 μg/mL rSEMA7A, 50 μM <t>RGDS</t> <t>peptide,</t> or both. Bar graph: ratio of pAKT to total AKT. Ordinary one-way ANOVA with Tukey’s multiple comparisons test. Error bars are mean +/- SD. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.
    Muscimol Tocris Bioscience Cat #0289, supplied by Tocris, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    A) Representative immunoblot for pAKT (S473), AKT and GAPDH in TC11 shCtrl and shSEMA7A (KD#1 and KD#2) cell lines. Bar graph: ratio of pAKT to total AKT. Ordinary one-way ANOVA with Tukey’s multiple comparisons test. B) Representative immunoblot for pAKT (S473), AKT and GAPDH in TC11 shCtrl and shSEMA7A KD#2 cell lines after treatment for 24 hours with 20 μg/mL purified rSEMA7A, or conditioned media from SEMA7A OE cells (S7OE CM). Bar graph: ratio of pAKT to total AKT. Ordinary one-way ANOVA with Tukey’s multiple comparisons test. C) Representative immunoblot for pAKT (S473), AKT and GAPDH in TC11 WT cell lysates after treatment for 1 hour with 20 μg/mL rSEMA7A, 50 μM RGDS peptide, or both. Bar graph: ratio of pAKT to total AKT. Ordinary one-way ANOVA with Tukey’s multiple comparisons test. Error bars are mean +/- SD. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.

    Journal: bioRxiv

    Article Title: Targeting Semaphorin 7a signaling in preclinical models of estrogen receptor-positive breast cancer

    doi: 10.1101/2025.05.21.655360

    Figure Lengend Snippet: A) Representative immunoblot for pAKT (S473), AKT and GAPDH in TC11 shCtrl and shSEMA7A (KD#1 and KD#2) cell lines. Bar graph: ratio of pAKT to total AKT. Ordinary one-way ANOVA with Tukey’s multiple comparisons test. B) Representative immunoblot for pAKT (S473), AKT and GAPDH in TC11 shCtrl and shSEMA7A KD#2 cell lines after treatment for 24 hours with 20 μg/mL purified rSEMA7A, or conditioned media from SEMA7A OE cells (S7OE CM). Bar graph: ratio of pAKT to total AKT. Ordinary one-way ANOVA with Tukey’s multiple comparisons test. C) Representative immunoblot for pAKT (S473), AKT and GAPDH in TC11 WT cell lysates after treatment for 1 hour with 20 μg/mL rSEMA7A, 50 μM RGDS peptide, or both. Bar graph: ratio of pAKT to total AKT. Ordinary one-way ANOVA with Tukey’s multiple comparisons test. Error bars are mean +/- SD. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.

    Article Snippet: For RGDS experiments, RGDS peptide (Tocris Bioscience; Cat#3498, 50uM in milliQ H 2 O) or control (milliQ H 2 O) was added to culture media for 1 hour prior to making cell lysates.

    Techniques: Western Blot, Purification